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pret flipsuc  (Addgene inc)


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    Structured Review

    Addgene inc pret flipsuc
    Expi293F cells co-expressing wildtype or variants of myc-tagged AtSWEET13 and sucrose FRET biosensor, <t>FlipSuc-90u</t> delta1, were incubated in Hank’s buffered saline solution, pH 7.4 (HBSS). At time point 40s, 40 mM sucrose in HBSS was added, and a negative ratio change was observed. Final solution concentration is equivalent to 20 mM sucrose. For the negative control, no significant change in the ratio was observed since cells expressed only the sucrose biosensor. Data are mean ± SEM, N = 3 biological replicates.
    Pret Flipsuc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pret flipsuc/product/Addgene inc
    Average 92 stars, based on 1 article reviews
    pret flipsuc - by Bioz Stars, 2026-04
    92/100 stars

    Images

    1) Product Images from "Deep mutational scanning reveals sequence to function constraints for SWEET family transporters"

    Article Title: Deep mutational scanning reveals sequence to function constraints for SWEET family transporters

    Journal: bioRxiv

    doi: 10.1101/2024.06.28.601307

    Expi293F cells co-expressing wildtype or variants of myc-tagged AtSWEET13 and sucrose FRET biosensor, FlipSuc-90u delta1, were incubated in Hank’s buffered saline solution, pH 7.4 (HBSS). At time point 40s, 40 mM sucrose in HBSS was added, and a negative ratio change was observed. Final solution concentration is equivalent to 20 mM sucrose. For the negative control, no significant change in the ratio was observed since cells expressed only the sucrose biosensor. Data are mean ± SEM, N = 3 biological replicates.
    Figure Legend Snippet: Expi293F cells co-expressing wildtype or variants of myc-tagged AtSWEET13 and sucrose FRET biosensor, FlipSuc-90u delta1, were incubated in Hank’s buffered saline solution, pH 7.4 (HBSS). At time point 40s, 40 mM sucrose in HBSS was added, and a negative ratio change was observed. Final solution concentration is equivalent to 20 mM sucrose. For the negative control, no significant change in the ratio was observed since cells expressed only the sucrose biosensor. Data are mean ± SEM, N = 3 biological replicates.

    Techniques Used: Expressing, Incubation, Saline, Concentration Assay, Negative Control



    Similar Products

    pret flipsuc  (Addgene inc)
    92
    Addgene inc pret flipsuc
    Expi293F cells co-expressing wildtype or variants of myc-tagged AtSWEET13 and sucrose FRET biosensor, <t>FlipSuc-90u</t> delta1, were incubated in Hank’s buffered saline solution, pH 7.4 (HBSS). At time point 40s, 40 mM sucrose in HBSS was added, and a negative ratio change was observed. Final solution concentration is equivalent to 20 mM sucrose. For the negative control, no significant change in the ratio was observed since cells expressed only the sucrose biosensor. Data are mean ± SEM, N = 3 biological replicates.
    Pret Flipsuc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pret flipsuc/product/Addgene inc
    Average 92 stars, based on 1 article reviews
    pret flipsuc - by Bioz Stars, 2026-04
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Expi293F cells co-expressing wildtype or variants of myc-tagged AtSWEET13 and sucrose FRET biosensor, FlipSuc-90u delta1, were incubated in Hank’s buffered saline solution, pH 7.4 (HBSS). At time point 40s, 40 mM sucrose in HBSS was added, and a negative ratio change was observed. Final solution concentration is equivalent to 20 mM sucrose. For the negative control, no significant change in the ratio was observed since cells expressed only the sucrose biosensor. Data are mean ± SEM, N = 3 biological replicates.

    Journal: bioRxiv

    Article Title: Deep mutational scanning reveals sequence to function constraints for SWEET family transporters

    doi: 10.1101/2024.06.28.601307

    Figure Lengend Snippet: Expi293F cells co-expressing wildtype or variants of myc-tagged AtSWEET13 and sucrose FRET biosensor, FlipSuc-90u delta1, were incubated in Hank’s buffered saline solution, pH 7.4 (HBSS). At time point 40s, 40 mM sucrose in HBSS was added, and a negative ratio change was observed. Final solution concentration is equivalent to 20 mM sucrose. For the negative control, no significant change in the ratio was observed since cells expressed only the sucrose biosensor. Data are mean ± SEM, N = 3 biological replicates.

    Article Snippet: The gene encoding the sucrose biosensor for fluorescence resonance energy transfer (FRET), FlipSuc-90u delta1, was subcloned from pRSET FlipSuc-90u delta1 into the BamHI-HindIII sites of pcDNA3.1(+). pRET FlipSuc-90u delta1 was a gift from Wolf Frommer (Addgene plasmid #14475; http://n2t.net/addgene:14475 ; RRID: Addgene_14475).

    Techniques: Expressing, Incubation, Saline, Concentration Assay, Negative Control